Test: Relative LOF error (%)

Add one or several tests for the relative lack-of-fit error to your assay. To add the tests as assay suitability tests, add them as children of the Assay suitability tests element. To add the tests as sample suitability tests, add them as children of the Sample suitability tests element.

Important: Test definitions are ignored without notification if you select a type of test that is not valid for the model you use or the scope you select.

To pass the test, the relative lack-of-fit error has to be less than or equal to the upper margin set in the test definition.

The relative lack-of-fit error is calculated as follows:

R = S N A - D ⋅ 100

Where S is the sum-of-squares lack of fit from the ANOVA table, N is the total number of observations, a is the upper asymptote, and d is the lower asymptote.

Supported models

4-parameter logistic, 3-parameter logistic with fixed lower asymptote, 3-parameter logistic with fixed upper asymptote

Assay elements

Test, Control

This element has the following attributes:

Data type Multiplicity Usage Default value
Node 0...unbounded optional none

Context:

/Quantitative response assay/Analysis/Suitability tests/Assay suitability tests/alternatives/

/Quantitative response assay/Analysis/Suitability tests/Sample suitability tests/alternatives/

/Test system definition (Quantitative response assay)/Suitability tests/Assay suitability tests/alternatives/

/Test system definition (Quantitative response assay)/Suitability tests/Sample suitability tests/alternatives/