A–D

Find reference information on document elements of the Dose-Response Analysis Package, letters A to D.

Activated

Select the Deactivated option from the drop-down list to disable Replicate averaging.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Empty 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Replicate averaging/choice/

Additional calculations

This section allows you to define additional calculations, for example, to report Effective concentrations or a Transduction unit.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_AdditionalCalc 0...1 optional none

Context:

/Dose-response analysis/Analysis/

Adjustment

Adjust your response values by Fixed value or by Assay element.

Expand the node to add or view information.

Example: Use this feature to subtract the absorbance of blank wells.

Tip: Add a scope element from the Creatable elements pane if you require.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 0...unbounded optional none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/

All sequence steps

Uses the full dose range for concentration calculation.

Tip: Select the Range partial option from the drop-down list if you do not want to use the full range.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Empty 1...1 required none

Context:

/Dose-response analysis/Analysis/Data selection schemes/Data selection scheme/choice/

Alpha

Select the alpha value you require for this test (alpha = 1 - significance level of the test).

Note: Higher values of alpha make the test less sensitive.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Alpha 1...1 required none

Context:

/Dose-response analysis/Analysis/Data selection schemes/Data selection scheme/choice/Dixon test/

/Dose-response analysis/Analysis/Data selection schemes/Data selection scheme/choice/Grubbs test/

/Dose-response analysis/Analysis/Data selection schemes/Data selection scheme/choice/(Externally) studentized residuals test/

Amount

Enter the amount of the substance.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:token 0...1 optional none

Context:

/Dose-response analysis/Documentation/Reagent/

Amount of the source material

This section allows you to specify the amount of source material to be used.

Expand the node to add or view information.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Dose-response analysis/Setup/Assay elements/Standard/choice/Concentration: Defined by source material/

/Dose-response analysis/Setup/Assay elements/Test/choice/Concentration: Defined by source material/

/Dose-response analysis/Setup/Assay elements/Control/choice/Concentration: Defined by source material/

Analysis

This section allows you to specify the Analytical model (regression), Response data processing (adjustments, normalizations, transformation), Data selection schemes (dose and response ranges, outlier detection), and Suitability tests (statistical tests) to be used in calculations.

Expand the node to add or view information.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Dose-response analysis/

Analysis purpose

Enter text that describes the purpose of this analysis.

Provide the analyte (target of the analysis), for example, and its unique identification.

Tip: You can enter multiline text.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 0...1 optional none

Context:

/Dose-response analysis/Documentation/

Analyte quantity per amount unit

This section allows you to specify the quantity of analyte contained in one unit of source material.

Expand the node to add or view information.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Dose-response analysis/Setup/Assay elements/Standard/choice/Concentration: Defined by source material/

/Dose-response analysis/Setup/Assay elements/Test/choice/Concentration: Defined by source material/

/Dose-response analysis/Setup/Assay elements/Control/choice/Concentration: Defined by source material/

Analytical model

This section allows you to specify the analytical model to be used for all assay elements.

Note: The system fits a regression curve for Muli-dose samples and interpolates a dose value for Single-dose samples.
Tip: Add the Additional calculations section from the Creatable elements pane to report Effective concentrations or a Transduction unit.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_AnalyticalModel 1...1 required none

Context:

/Dose-response analysis/Analysis/

Anova terms

This element has the following attributes:

Data type Multiplicity Usage Default value
L_AdditionalTests 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Margin development/

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/

Assay element

Select the assay element to be used for calculating the adjustment value.

Tip: Add the Sequence step element from the Creatable elements pane if you want to use a particular sequence step of the assay element.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Adjustment/choice/by Assay element/

Assay element

Select the assay element to be used for calculating the normalization factor.

Tip: Add the Sequence step element from the Creatable elements pane if you want to use a particular sequence step of the assay element.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:token 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Normalization/choice/by Assay element/

Assay element scope

Select the assay element whose response values are to be modified during response data processing.

Tip: If you require 'Assay element type scope', select the corresponding option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 0...1 optional none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Transformation/choice/

Assay element scope

Select the assay element you want to include in the test.

Tip: If you want to include an entire assay element type rather than just a particular assay element, select the 'Assay element type scope' option from the left drop-down list.
Note: The test type also influences which assay elements are included in a test. Some tests are only performed for particular types of assay elements. Moreover, the model determines which tests are feasible. Some test definitions may not work with your model. Please consult the help pages on particular tests for additional information.
Important: Test definitions are ignored without notification if the Scope you select results in invalid test definitions (for example, if a test cannot be performed for the assay element you select).

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 1...1 optional none

Context:

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/F-test (hypothesis test)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Equivalence test (parameter estimate)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Parameter/property point estimate/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Mean squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Sum of squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Value of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Interval of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Width of interpolation interval/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Maximal number of outliers/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Maximal sequence step CV(%)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Coefficient of determination (R²)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Response value range/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Test on effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Response at effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Transduction unit/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Recovery rate/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/F-test (hypothesis test)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Equivalence test (parameter estimate)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Parameter/property point estimate/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Mean squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Sum of squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Value of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Interval of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Width of interpolation interval/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Maximal number of outliers/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Maximal sequence step CV(%)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Coefficient of determination (R²)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Response value range/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Test on effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Response at effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Transduction unit/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Recovery rate/choice/

Assay element scope

Select the assay element whose response values are to be modified during response data processing.

Tip: If you require 'Assay element type scope' or 'Plate scope', select the corresponding option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 0...1 optional none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Adjustment/choice/

/Dose-response analysis/Analysis/Response data processing/choice/Normalization/choice/

Assay element type scope

Select the assay element type whose response values are to be modified during response data processing.

The following options are available: All assay elements, Standard only, Test samples only, Control samples only.

Tip: If you require 'Assay element scope', select the corresponding option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Scope 0...1 optional none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Transformation/choice/

Assay element type scope

Select the assay element type you want to include in the test.

The following options are available from the drop-down list:

  • All assay elements: Runs the test for every assay element.
  • Standard only: Runs the test for the Standard sample.
  • Test samples only: Runs the test for every Test sample.
  • Control samples only: Runs the test for every Control sample.
Note: The test type also influences which assay elements are included in a test. Some tests are only performed for particular types of assay elements. Moreover, the model determines which tests are feasible. Some test definitions may not work with your model. Please consult the help pages on particular tests for additional information.
Important: Test definitions are ignored without notification if the Scope you select results in invalid test definitions (for example, if a test cannot be performed for the assay element type you select).

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Scope 1...1 optional none

Context:

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/F-test (hypothesis test)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Equivalence test (parameter estimate)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Parameter/property point estimate/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Mean squares (ANOVA)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Sum of squares (ANOVA)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Value of interpolation/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Interval of interpolation/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Width of interpolation interval/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Maximal number of outliers/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Maximal sequence step CV (%)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Coefficient of determination (R²)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Response value range/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Effective concentration/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Response at effective concentration/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Transduction unit/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Test: Recovery rate/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/F-test (hypothesis test)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Equivalence test (parameter estimate)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Parameter/property point estimate/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Mean squares (ANOVA)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Sum of squares (ANOVA)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Value of interpolation/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Interval of interpolation/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Width of interpolation interval/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Maximal number of outliers/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Maximal sequence step CV (%)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Coefficient of determination (R²)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Response value range/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Effective concentration/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Response at effective concentration/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Transduction unit/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Test: Recovery rate/

Assay element type scope

Select the assay element type you want to include in the test.

The following options are available from the drop-down list:

  • All assay elements: Runs the test for every assay element.
  • Standard only: Runs the test for the Standard sample.
  • Test samples only: Runs the test for every Test sample.
  • Control samples only: Runs the test for every Control sample.
Tip: If you just want to include a particular assay element rather than an entire assay element type, select the 'Assay element scope' option from the left drop-down list.
Note: The test type also influences which assay elements are included in a test. Some tests are only performed for particular types of assay elements. Moreover, the model determines which tests are feasible. Some test definitions may not work with your model. Please consult the help pages on particular tests for additional information.
Important: Test definitions are ignored without notification if the Scope you select results in invalid test definitions (for example, if a test cannot be performed for the assay element type you select).

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Scope 1...1 optional none

Context:

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/F-test (hypothesis test)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Equivalence test (parameter estimate)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Parameter/property point estimate/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Mean squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Sum of squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Value of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Interval of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Width of interpolation interval/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Maximal number of outliers/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Maximal sequence step CV(%)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Coefficient of determination (R²)/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Response value range/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Test on effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Response at effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Transduction unit/choice/

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Test: Recovery rate/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/F-test (hypothesis test)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Equivalence test (parameter estimate)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Parameter/property point estimate/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Mean squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Sum of squares (ANOVA)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Value of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Interval of interpolation/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Width of interpolation interval/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Maximal number of outliers/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Maximal sequence step CV(%)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Coefficient of determination (R²)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Response value range/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Test on effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Response at effective concentration/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Transduction unit/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Test: Recovery rate/choice/

Assay element type scope

Select the assay element type whose response values are to be modified during response data processing.

The following options are available from the drop-down list:

  • All assay elements
  • Standard only
  • Test samples only
  • Control samples only
Tip: If you require 'Assay element scope' or 'Plate scope', select the corresponding option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Scope 0...1 optional none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Adjustment/choice/

/Dose-response analysis/Analysis/Response data processing/choice/Normalization/choice/

Assay elements

This section allows you to define all assay elements.

Expand the node to add or view information.

Dose-response analysis documents contain three types of assay elements: The Standard sample, Test samples, and Control samples. Standard and Test samples are diluted samples. Control samples with single-dose preparation schemes may also serve as positive, negative, or blank controls.

Add one or several of the following assay elements from the context menu or Creatable elements pane:

  • Test: To determine their relative concentrations.
  • Control: To define additional acceptance criteria.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_AssayElements 1...1 required none

Context:

/Dose-response analysis/Setup/

Assay suitability tests

Add the Assay suitability tests you require.

Important: The results of Assay suitability tests affect the whole assay rather than just the individual assay elements that are evaluated.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SuitabilityTestsForDra 1...1 required none

Context:

/Dose-response analysis/Analysis/Suitability tests/

/Test system for dose-response analysis/Suitability tests/

Asymmetry parameter (G) (nonlinear models)

Use this test for the following models:

  • 5-parameter logistic fit

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SingleTestStrategy 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Test: Parameter/property point estimate/

/Equivalence margin development for dose-response analysis assays/Margin development/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Margin development/Test: Parameter/property point estimate/

Asymptote fixed by test sample or control sample

Please select the assay element you want to use to calculate the asymptote.

Note: The mean of the response values of the test or control sample you select is used as value of the fixed asymptote.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 1...1 required none

Context:

/Dose-response analysis/Analysis/Analytical model/choice/Model: 3-parameter logistic fit with fixed upper asymptote/choice/

/Dose-response analysis/Analysis/Analytical model/choice/Model: 3-parameter logistic fit with fixed lower asymptote/choice/

Asymptote fixed by value

Enter the number to be used as value of the fixed asymptote.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:double 1...1 required none

Context:

/Dose-response analysis/Analysis/Analytical model/choice/Model: 3-parameter logistic fit with fixed upper asymptote/choice/

/Dose-response analysis/Analysis/Analytical model/choice/Model: 3-parameter logistic fit with fixed lower asymptote/choice/

Author

Automatically inserts the user name of the current user when the Author element is created. You can enter a different name.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:token 1...1 required none

Context:

/Dose-response analysis/Comment/

/Test system for dose-response analysis/Comment/

B parameter (nonlinear models)

Use this test for the following models:

  • 4-parameter logistic fit
  • 4-parameter logistic fit with included blanks
  • 5-parameter logistic fit

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SingleTestStrategy 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Test: Parameter/property point estimate/

/Equivalence margin development for dose-response analysis assays/Margin development/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Margin development/Test: Parameter/property point estimate/

Base

Enter a number to specify the Base of this sequence.

Note: The number you enter must be a positive decimal.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_PositiveDouble 1...1 required none

Context:

/Dose-response analysis/Setup/Preparation schemes/Multi-dose scheme/choice/Geometric sequence/

by Assay element

Select an option from the 'Assay element' drop-down list below to specify the Assay element to be used for calculating the adjustment value.

Note: By default, the adjustment you select is applied every response value. Add a scope from the Creatable elements pane to restrict its application to an Assay element type, Assay element, or Plate.
Tip: If you require 'No adjustment' or adjustment 'by Fixed value', select the corresponding option from the 'Adjustment' drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Adjustment/choice/

by Assay element

Select an option from the 'Assay element' drop-down list below to specify the Assay element to be used for calculating the normalization value.

Note: By default, the normalization you select is applied to every response value. Add a scope from the Creatable elements pane to restrict its application to an Assay element type, Assay element, or Plate.
Tip: If you require 'No normalization' or normalization 'by Fixed value', select the corresponding option from the 'Normalization' drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Normalization/choice/

by Fixed value

Enter a number to specify the Fixed value to be subtracted from your response values.

Note: By default, the number you enter is subtracted from every response value. Add a scope from the Creatable elements pane to restrict its application to an Assay element type, Assay element, or Plate.
Tip: If you require 'No adjustment' or adjustment 'by Assay element', select the corresponding option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:double 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Adjustment/choice/

by Fixed value

Enter a number to specify the Fixed value by which your response values are to be divided.

Note: By default, every response value is divided by the number you enter. Add a scope from the Creatable elements pane to restrict its application to an Assay element type, Assay element, or Plate.
Tip: If you require 'No normalization' or normalization 'by Assay element', select the corresponding option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:double 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Normalization/choice/

C parameter (nonlinear models)

Use this test for the following models:

  • 4-parameter logistic fit
  • 4-parameter logistic fit with included blanks
  • 5-parameter logistic fit

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SingleTestStrategy 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Test: Parameter/property point estimate/

/Equivalence margin development for dose-response analysis assays/Margin development/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Margin development/Test: Parameter/property point estimate/

Column: Boolean

Define a column that allows you to associate each observation with a Boolean value.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_BooleanColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Date

Define a column that allows you to associate each observation with a date.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_DateColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Date/time

Define a column that allows you to associate each observation with a date and time.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_DateTimeColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Document reference

Expand the node to add or view information.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_ReferenceColumn 0...1 optional none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Floating-point number

Define a column that allows you to associate each observation with a value of double-precision floating-point number format.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_DoubleColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Integer

Define a column that allows you to associate each observation with a value of integer number format.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_IntegerColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column key

Currently, no information available.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:token 1...1 required none

Context:

/Equivalence margin development for dose-response analysis assays/Datatable/Column: Assay element/

Column: Memo (multiline text)

Define a column that allows you to associate each observation with a multiline text.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_MemoColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Observation group

Links individual observations (measurements, data table rows) to their assay element (sample).

Each observation group comprises the observations of a single assay element (sample). The unique ID of the observation group is set in the definition of the assay element.

This data table column is named Observation group ID by default. It displays both the observation group ID and the assay element name for each observation.

Note: The Maximal group number limits the number of observation groups (assay elements) for which the Observations editor can display data. If your assay document has more assay elements, you require more observation groups and must change the Maximal group number accordingly.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_ObservationGroupColumn 1...1 required none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Position factor

Define up to three columns that allow you to associate each observation with the position where the observation occurred.

If you work with a microplate, for example, define two position factor columns to specify the microplate row and column of each observation. If you work with several microplates, define a third position factor column to provide the plate number of each observation.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_PositionColumn 0...3 optional none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Response value

Holds the response values of individual observations.

You can acquire the values using one of our Data Acquisition Modules, copy and paste them from an external application such as a spreadsheet, or type them in manually.

This data table column is named Response by default.

Note: The data type of this column is double-precision floating-point number.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_ExtendedResponseFactor 1...1 required none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Section

Expand the node to add or view information.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_ReferenceSectionColumn 0...1 optional none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Sequence step

Links individual observations (measurements, data table rows) to their dilution sequence step.

The step number is implied by the sequence settings in the preparation scheme of the assay element (sample).

This data table column is named Sequence step by default. It displays both the sequence step number and the dose value for each observation.

Note: The total number of steps and replicates of an assay element is defined in the preparation scheme referenced by this assay element.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_SequenceStepColumn 1...1 required none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Technical-outlier flag

Provides a column to flag technical outliers in the data table.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_TechnicalOutlierColumn 1...1 required none

Context:

/Dose-response analysis/Setup/Observation data/

Column: Text

Define a column that allows you to associate each observation with a single-line text.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_TextColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Time

Define a column that allows you to associate each observation with a time.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_TimeColumn 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Observation data/choice/

Column: Well ID

Define a column that allows you to associate each observation with a well ID.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_WellIdColumn 0...1 optional none

Context:

/Dose-response analysis/Setup/Observation data/

Comment

Enter a comment, or add a reference to a comment document.

Tip: You can add several Comment elements. And to each Comment element, you can add a Subject element, Text elements, and Linked document elements.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Comment 0...unbounded optional none

Context:

/Dose-response analysis/

Comment

Enter a comment, or add a reference to a comment document.

Tip: You can add several Comment elements. And to each Comment element, you can add a Subject element, Text elements, and Linked document elements.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Comment 0...unbounded optional none

Context:

/Test system for dose-response analysis/

Concentration: Defined by source material

Define the concentration of this assay element based on the source material.

Expand the node to add or view information.

This definition serves to calculate either of the following:

  • The first dose of the dilution sequence of this assay element if you do not enter a value for First dose in the Preparation scheme of this assay element.
  • An implicit predilution factor if you provide a value for First dose in the Preparation scheme.
Note: The units you enter here also apply to the x-axis of the graph unless you provide the First dose in the Preparation scheme.
Tip: You can define the concentration based on the stock solution instead of the source material. Select the 'Concentration: Defined by stock solution' option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 0...1 optional none

Context:

/Dose-response analysis/Setup/Assay elements/Standard/choice/

/Dose-response analysis/Setup/Assay elements/Test/choice/

/Dose-response analysis/Setup/Assay elements/Control/choice/

Concentration: Defined by stock solution

Define the concentration of this assay element based on the stock solution.

Expand the node to add or view information.

This definition serves to calculate either of the following:

  • The first dose of the dilution sequence of this assay element if you do not enter a value for First dose in the Preparation scheme of this assay element.
  • An implicit predilution factor if you provide a value for First dose in the Preparation scheme.
Note: The units you enter here also apply to the x-axis of the graph unless you provide the First dose in the Preparation scheme.
Tip: You can define the concentration based on the source material instead of the stock solution. Select the 'Concentration: Defined by source material' option from the drop-down list.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 0...1 optional none

Context:

/Dose-response analysis/Setup/Assay elements/Standard/choice/

/Dose-response analysis/Setup/Assay elements/Test/choice/

/Dose-response analysis/Setup/Assay elements/Control/choice/

Confidence level

Applies to margin development with tests that use confidence intervals. Select the Confidence level for which margins are to be calculated. A common value is 90%.

Note: A higher Confidence level produces broader confidence intervals, which results in a greater equivalence range (distance between equivalence margins).
Note: This setting has no effect if you work with tests without confidence intervals such as point estimate tests.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_ConfidenceLevel 1...1 required none

Context:

/Equivalence margin development for dose-response analysis assays/Settings/

Confidence level (%)

Select the Confidence level of the test. A common value is 95%.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_ConfidenceLevel 1...1 required none

Context:

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/Equivalence test (parameter estimate)/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/Equivalence test (parameter estimate)/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/Equivalence test (parameter estimate)/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/Equivalence test (parameter estimate)/

Data type Multiplicity Usage Default value
T_ConfidenceLevel 0...1 required none

Context:

/Dose-response analysis/Analysis/Suitability tests/Assay suitability tests/choice/F-test (hypothesis test)/choice/

/Dose-response analysis/Analysis/Suitability tests/Sample suitability tests/choice/F-test (hypothesis test)/choice/

/Test system for dose-response analysis/Suitability tests/Assay suitability tests/choice/F-test (hypothesis test)/choice/

/Test system for dose-response analysis/Suitability tests/Sample suitability tests/choice/F-test (hypothesis test)/choice/

Control

This section allows you to provide information on a Control sample.

Expand the node to add or view information.

Note: The system treats Control samples in the same way as Test samples. Every Control sample has a Preparation scheme which can be of two types, that is, Multi-dose scheme or Single-dose scheme.
  • For Control samples that rely on Multi-dose schemes, a regression is performed during calculation.
  • For Control samples that rely on Single-dose schemes, a dose value is interpolated using the regression curve of the Standard sample.
Tip: To add a control line to your assay, set up a Control sample based on a Single-dose scheme.
Tip: Suitability tests allow you to set a test scope based on assay element type such as Control samples only.
Tip: To provide information on the substance: Add the elements you need, such as Substance name or Substance reference, from the Creatable elements pane.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_AE_Control 0...unbounded optional none

Context:

/Dose-response analysis/Setup/Assay elements/

Covariance of A upper asymptote and D lower asymptote

Enter a decimal number to be used as covariance of the upper asymptote and the lower asymptote. Simulation uses it for testing sampled parameter configurations. If you do not enter a value, 0 is used as default.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:double 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Visualization/Simulation space/

Creation time

Currently, no information available.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:dateTime 0...1 optional none

Context:

/Test system for dose-response analysis/

Data selection scheme

Assign a Data selection scheme to this assay element.

Data selection schemes contain settings on outlier detection and on dose and response range selection.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 1...1 required none

Context:

/Dose-response analysis/Setup/Assay elements/Standard/

/Dose-response analysis/Setup/Assay elements/Test/

/Dose-response analysis/Setup/Assay elements/Control/

Data selection scheme

This section allows you to define a Data selection scheme. Data selection schemes contain settings on outlier detection and on dose and response range selection.

Expand the node to add or view information.

Note: In every assay element, you must reference exactly one Data selection scheme. You can reference any Data selection scheme from more than one assay element.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...unbounded required none

Context:

/Dose-response analysis/Analysis/Data selection schemes/

Data selection schemes

This section allows you to define multiple data selection schemes for this assay. Data selection schemes contain settings on outlier detection and on dose and response range selection.

Expand the node to add or view information.

Note: In every assay element, you must reference exactly one Data selection scheme. You can reference any Data selection scheme from more than one assay element.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_DataSelectionSchemes 1...1 required none

Context:

/Dose-response analysis/Analysis/

Data source

This section provides information on imported data.

Expand the node to add or view information.

Note: Import information provided in this section was supplied by the import module.
Tip: Add an Import comment element to provide additional information.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 0...unbounded optional none

Context:

/Dose-response analysis/Documentation/

Data table

This section allows you to adjust the dataset structure used to calculate the margins. Expand the node to add or view information.

Note: Much of the dataset structure is set automatically and not visible in the Content editor.
Tip: View the data table in the References editor.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Equivalence margin development for dose-response analysis assays/

Date

Automatically inserts the current date and time when the Date element is created. You can enter a different date and time.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:dateTime 1...1 required none

Context:

/Dose-response analysis/Comment/

/Test system for dose-response analysis/Comment/

Date

Automatically inserts the current date and time when the document is created. You can enter a different date and time.

Tip: Use the date for sorting in aggregation documents.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:dateTime 1...1 required none

Context:

/Equivalence margin development for dose-response analysis assays/

Date

Automatically inserts the current date and time when the document is created. You can enter a different date and time.

Tip: Use the date for sorting in aggregation documents.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:dateTime 1...1 required none

Context:

/Dose-response analysis/

Date

Automatically inserts the current date and time when the document is created. You can enter a different date and time.

Tip: Use the date for sorting in aggregation documents.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:dateTime 1...1 required none

Context:

/Test system for dose-response analysis/

Deactivated

Select the Activated option from the drop-down list to enable Replicate averaging.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Empty 1...1 required none

Context:

/Dose-response analysis/Analysis/Response data processing/choice/Replicate averaging/choice/

Degrees of freedom

Enter an integer to be used as degrees of freedom of the t-distribution in visualization.

Note: This value only applies if no assays are referenced. Otherwise, the degrees of freedom of the referenced assays are used.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:positiveInteger 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Visualization/

Delta

Enter a number to specify the difference between the dose values of this sequence.

Note: The number you enter must be a non-negative decimal.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:double 1...1 required none

Context:

/Dose-response analysis/Setup/Preparation schemes/Multi-dose scheme/choice/Linear sequence/

Development assay

Marks dataset rows as development assays. Rows marked as development assays (value set to 'true') are used to derive margins.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_DevelopmentData 1...1 required none

Context:

/Equivalence margin development for dose-response analysis assays/Datatable/

Difference of asymptotes (A - D) (nonlinear models)

Use this test for the following models:

  • 4-parameter logistic fit
  • 4-parameter logistic fit with included blanks
  • 5-parameter logistic fit

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SingleTestStrategy 0...1 optional none

Context:

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Test strategies/Strategy/Test: Parameter/property point estimate/

/Equivalence margin development for dose-response analysis assays/Margin development/Equivalence test (parameter estimate)/

/Equivalence margin development for dose-response analysis assays/Margin development/Test: Parameter/property point estimate/

Dilution factor

Enter a number to set the Dilution factor for an individual step in the dilution sequence. The concentration of this step is equal to the concentration of the previous step divided by the dilution factor.

Tip: Add additional Dilution factor elements as you require.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_PositiveDouble 1...unbounded required none

Context:

/Dose-response analysis/Setup/Preparation schemes/Multi-dose scheme/choice/Stepwise dilution sequence/

Display color

Select the color to be used to display data of this assay element in graphs and tables. When you create an assay element, a color is automatically assigned. Select the color field to display controls that allow you to change the color.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:string 1...1 required none

Context:

/Dose-response analysis/Setup/Assay elements/Standard/

/Dose-response analysis/Setup/Assay elements/Test/

/Dose-response analysis/Setup/Assay elements/Control/

Display horizontal lines

Select the 'yes' option from the drop-down list to add horizontal grid lines to your plots.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SSY_Boolean 1...1 required none

Context:

/Dose-response analysis/Settings/Graphics/

Display vertical lines

Select the 'yes' option from the drop-down list to add vertical grid lines to your plots.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_SSY_Boolean 1...1 required none

Context:

/Dose-response analysis/Settings/Graphics/

Dixon test

Provides a statistical test for the detection of data outliers based on: Dixon, Wilfrid J. 1953. 'Processing data for outliers.' Biometrics 9 (1): 74-89.

The test is based on replicates. Sensitivity is defined by the respective alpha value.

Note: Our Dixon test implements corrections concerning the calculation of critical values based on Rorabacher.* Yet, for reasons of compatibility, we use the alpha level provided in the original Dixon paper where the test is defined as one-sided test. The significance level of the corresponding two-sided test is alpha/2.

The Dixon test is also described in the United States Pharmacopeia: USP 28 NF 23 (2005), p. 2521.

* Rorabacher, David B. 1991. 'Statistical treatment for rejection of deviant values: Critical values of Dixon's "Q" parameter and related subrange ratios at the 95% confidence level.' Analytical Chemistry 63 (2): 139–146.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

/Dose-response analysis/Analysis/Data selection schemes/Data selection scheme/choice/

Document reference

Configure the document reference column. Expand the node to add or view information.

This element has the following attributes:

Data type Multiplicity Usage Default value
ds:T_ReferenceColumn 1...1 required none

Context:

/Equivalence margin development for dose-response analysis assays/Datatable/

Documentation

This section allows you to provide information on operators, analyte, reagents, and equipment.

To provide information on the substance: Add the elements you need, such as Substance name or Substance reference, from the Creatable elements pane.

This element has the following attributes:

Data type Multiplicity Usage Default value
T_Documentation 0...1 optional none

Context:

/Dose-response analysis/

Dose-response analysis

Dose-response analysis documents provide the features you need for curve comparisons and calibration curves.

Navigate through the properties to select or enter settings you require for this Dose-response analysis:

1. Expand the node to add or view information.

  • The second level of the document structure displays the Name and Date of the document.
  • It also provides access to the Documentation, Setup, and Analysis sections.

2. Double-click the Identification element in the Creatable elements pane to add it to the document.

Note: To delete an element from the document, right-click the element in the document structure and select the Delete element option from the pop-up menu.

3. Double-click the Comment or Settings element in the Creatable elements pane to add a Comment or Settings section to the document.

This element has the following attributes:

Data type Multiplicity Usage Default value
xs:empty 1...1 required none

Context:

Top level