Dose-response analysis
Use Dose-response analysis documents to perform curve comparisons and create calibration curves.
About dose-response analysis
Dose-response analysis documents support the evaluation of biological assays by modeling the relationship between concentration and response. The analysis is based on regression models that describe how the biological response changes with varying doses of a treatment.
By fitting a curve to the response data, you can interpolate unknown sample concentrations or compare assay performance using key model parameters. Dose-response analysis is commonly used in potency determination, assay validation, and stability studies.
PLA 3.0 provides a range of tools that support you in configuring the analysis according to your assay design. This includes defining preparation schemes, selecting regression models, adjusting response data, and applying suitability tests to validate your results.
About this document type
Dose-response analysis documents offer the features you need for the analysis of dose-response curves. They support the calculation of substance concentration, effective concentration, recovery rates, and curve characteristics.
You can configure any number of diluted and undiluted samples, such as Standard, Tests, and Controls, and apply linear or logistic models (4PL, 5PL, 3PL, and 2PL) or hill equation for curve fitting. Dose-response analysis documents also let you define suitability tests to ensure assay validity.
icon in the
upper right corner of the Content editor.Document structure
Each Dose-response analysis document has three default sections, that is, Documentation, Setup, and Analysis, plus an optional Settings and one or more Comment sections.
| Section | Description |
|---|---|
|
Documentation |
Provide supplemental information on the setup of your assay that is needed for documentary purposes but is not required for calculation or the organization of observation data. |
|
Setup |
Set up the layout of your assay with assay elements, preparation schemes, and observation data. |
|
Analysis |
Lets you to define how to perform the statistical analysis. |
|
Settings |
Define how you want to graphically display assay results. |
|
Comment |
Add extra information to your assay. The PDF reports display the comments you add on the first page. |
Get started
To get started efficiently, we recommend becoming familiar with the concepts that make up the document structure, as they form the foundation for a structured and reliable evaluation in PLA 3.0. For details and best practices, see the Concepts for efficient setup topic.