Test: Relative LOF error (%)
Add one or several tests for the relative lack-of-fit error to your assay.
Overview
To add the tests as assay suitability tests, add them as children of the Assay suitability tests element. To add the tests as sample suitability tests, add them as children of the Sample suitability tests element.
To pass the test, the relative lack-of-fit error has to be less than or equal to the upper margin set in the test definition.
The relative lack-of-fit error is calculated as follows:
Where S is the sum-of-squares lack of fit from the ANOVA table, N is the total number of observations, a is the upper asymptote, and d is the lower asymptote.
Supported models
4-parameter logistic, 3-parameter logistic with fixed lower asymptote, 3-parameter logistic with fixed upper asymptote
Assay elements
Test, Control