Define your plate layout

Define how your samples are arranged on your plate.

Before you begin

You have defined the analytical settings.

About this task

Defining plate layouts for bioMérieux endotoxin detection assay involves three steps. First, you assign assay elements to wells. Next, you assign dilutions or sequence steps to wells. Then, you assign positive product control to wells.

To perform these assignments, you use the primary display factor drop-down list () in the toolbar of the By Position editor. For a detailed description of this editor, see the By Position editor topic.

Tip: To improve the readability of your table, you can activate the grid color view option. This use case uses the By initial response grid coloring.

Procedure

To assay elements to wells:
  1. In the By Position editor, select Observation group ID as the primary display factor. Observation group IDs link assay elements to their respective observation data.
  2. Assign samples to wells according to the following logic:
    Wells Observation Group ID

    B1, B2, C1, C2, D1, D2

    [1] Standard: STD

    A3, B3, C3, D3

    [2] Test: PureW1

    A4, B4, C4, D4

    [3] Test: PureW2

    A5, B5, C5, D5

    [4] Test: PureW3

    A6, B6, C6, D6

    [5] Test: WIF1

    A7, B7, C7, D7

    [6] Test: WIF2

    A8, B8, C8, D8

    [7] Test: WIF3

    A9, B9, C9, D9

    [8] Test: Val1

    A10, B10, C10, D10

    [9] Test: Val2

    A11, B11, C11, D11

    [10] Test: Val3

    F1, F2, G1, G2

    [11] Blank control
    Result: Your plate layout should now look like this:
    Figure 1. Assignment of assay elements to wells in the By Position editor
To assign sequence steps to wells:
  1. Select Sequence step as the primary display factor.
  2. Assign sequence steps to wells according to the following logic:
    Wells Sequence step

    B1 and B2

    [1] 5

    C1 and C2

    [2] 0,5

    D1 and D2

    [3] 0,05

    A4 to A11

    [1] <undefined>

    B4 to B11

    [1] <undefined>

    C4 to C11

    [1] <undefined>

    D4 to D11

    [1] <undefined>

    F1 and F2

    [1] <undefined>

    G1 and G2

    [1] <undefined>
    Result: Your plate layout should now look like this:
    Figure 2. Assignment of sequence steps to wells in the By Position editor
To assign positive positive product control to wells:
  1. Select Positive product control as the primary display factor.
  2. Set the setting to 'true' for wells A3 to A 11, and B3 to B11.
    Result: Your plate layout should now look like this:
    Figure 3. Assignment of positive product control to wells in the By Position editor
  3. Save the document.

What to do next

Enter your observation data.