Preparation schemes

Preparation schemes correspond to industry-standard practices of dose preparation protocols. They let you efficiently assign dilution sequences and replicate settings to assay elements, ensuring consistency and reducing redundant input during setup.

Note: Each assay element must have a preparation scheme assigned. And each Dichotomous assay document must contain at least one preparation scheme.

Properties

Use the following Preparation scheme properties to set up dilution sequences:
  • Use the Replicate count to specify how many replicates an assay element requires for each dose step.

  • Use Predilution factors to express how much of the original stock solution is present in the total solution.

  • Set up the dilution sequence manually by defining each step (Defined sequence or Stepwise dilution sequence), or define a set of variables to calculate the dilution sequence based on these variables (Geometric sequence or Linear sequence).

  • Optionally, you can also define the absolute potency by stock solution or by raw material, and the unit for the absolute potency you want to use in reporting.

Example

Assume that you want to develop an assay whose assay elements have five dose steps and one replicate. The dilution sequence is calculated as a 2.0-fold geometric sequence. You therefore set up the Preparation scheme with a Replicate count of 1, a Geometric sequence with 5 as the Number of steps.