New Cylinder plate assay (USP <81>) documents have a single test sample. If you add
extra test samples, you also have to adjust the position factors.
Before you begin
Create a Cylinder plate assay (USP <81>) document.
About this task
By default, Cylinder plate assay (USP <81>) documents contain a single test sample and
15 plates. This covers 3 plate replicates of the following:
The standard has a 5-step concentration sequence and therefore requires
12 plates (Petri dishes), that is, 3 plate replicates for each of the 4
concentration steps.
The test sample requires 3 plates.
You can view the default setup in the By
Position editor. The data table has 6 rows (cylinders per plate) and
15 columns (plates). 3 observation group IDs are assigned.Figure 1. Default setup of a Cylinder plate assay (USP <81>) document displayed in
the By Position editor (cropped)
In this example, you add an extra test sample with three plate replicates. Therefore,
you have to increase the number of plates by three, that is, from 15 to 18.
Procedure
To add a test sample:
In the Content editor, go to Setup > Assay elements.
Use the Creatable elements pane to add an extra
Test element.
Result: PLA 3.0 adds a test sample named TST
2 to the Assay elements
section.
To adjust the position factors:
Go to Setup > Observation data.
Go to the position factor for the plate (second Column: Position
factor element) and expand it.
For the Number of positions element, change the value
from 15 (default) to 18.
Save the document.
Result
In the By Position editor, the data table now has 18 columns
(plates). The fourth observation group ID is assigned to the test sample you created.
The number of rows (cylinders per plate) is the same as before.Figure 2. Setup of a Cylinder plate assay (USP <81>) document with 18 plates displayed
in the By Position editor (cropped)
